POLR1H
Domain
The TFIIS-type zinc-binding beta-ribbon domain contains an acidic hairpin motif (residues Asp-106, Glu-107) that likely coordinates the nucleophilic water and magnesium to cleave the scissile phosphodiester bond and release the mis-incorporated 5'-ribonucleotides.
Function
Core component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase which synthesizes ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates. Can mediate Pol I proofreading of the nascent RNA transcript. Anchors into the Pol I active site to monitor transcription fidelity and cleave mis-incorporated 5'-ribonucleotides.
Sequence Similarities
Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.
Cellular localization
- Nucleus
- Nucleolus
Alternative names
RPA12, ZNRD1, POLR1H, DNA-directed RNA polymerase I subunit RPA12, DNA-directed RNA polymerase I subunit H, Zinc ribbon domain-containing protein 1