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POU5F1

Developmental stage

Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.

Domain

The POU-specific domain mediates interaction with PKM.

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Post-translational modifications

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.

Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.

ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.

Sequence similarities

Belongs to the POU transcription factor family. Class-5 subfamily.

Tissue specificity

Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

Cellular localization

  • Cytoplasm
  • Nucleus
  • Expressed in a diffuse and slightly punctuate pattern. Colocalizes with MAPK8 and MAPK9 in the nucleus.

Alternative names

  • OCT3
  • OCT4
  • OTF3
  • OCT3
  • POU5F1
  • OCT4
  • OTF3
  • Octamer-binding protein 3
  • Octamer-binding protein 4
  • Octamer-binding transcription factor 3
  • Oct-3
  • Oct-4
  • OTF-3

Target type

Proteins

Primary research area

Epigenetics

Other research areas

  • Oncology

Molecular weight

38571Da

We found 35 products in 4 categories

Assay Kits

Target

Reactive species

Detection method

Proteins & Peptides

Target

Species of origin

Cell Lines & Lysates

Target

Cell type

Species or organism

Search our catalogue for 'POU5F1' (35)

Products

ab181557

Anti-Oct4 antibody [EPR17929] - ChIP Grade

Recombinant
RabMAb
Advanced Validation

ab200834

Anti-Oct4 antibody [EPR17980]

Recombinant
RabMAb
Advanced Validation

ab109183

Anti-Oct4 antibody [EPR2054]

Recombinant
RabMAb
Advanced Validation

ab215386

Anti-Oct4 antibody [EPR17929] - Low endotoxin, Azide free

Recombinant
RabMAb
Advanced Validation

ab240358

Anti-Oct4 antibody [EPR17980] - BSA and Azide free

Recombinant
RabMAb
Advanced Validation

ab235653

Human/Mouse Oct4 ELISA Kit

Recombinant
SimpleStep

ab222233

Anti-Oct4 antibody [EPR2054] - BSA and Azide free

Recombinant
RabMAb
Advanced Validation