POU5F1
GeneName
POU5F1
Summary
POU5F1, also known as OCT4 or Oct-4, is a 39 kDa transcription factor that plays a crucial role in maintaining pluripotency and self-renewal of embryonic stem cells. It is primarily expressed in the nucleus and cytoplasm of stem cells, where it binds to specific DNA sequences to regulate gene expression. POU5F1 is involved in various biological processes, including blastocyst development, cardiac cell fate determination, and the regulation of transcription by RNA polymerase II. Additionally, it interacts with other proteins to influence cell fate commitment and morphogenesis, highlighting its importance in early development and stem cell biology.
Importance
POU5F1 is relevant to: - Stem cell research due to its role in maintaining pluripotency and self-renewal in embryonic stem cells - Developmental biology, particularly in understanding the mechanisms of early embryogenesis and cell differentiation - Cancer research, as aberrant expression of POU5F1 has been associated with various malignancies and tumour progression - Regenerative medicine, where manipulating POU5F1 expression could enhance stem cell therapies and tissue regeneration.
Top Products
For researchers investigating POU5F1, we recommend two excellent primary antibodies. The first is the well-cited polyclonal antibody, Anti-Oct4 antibody (ab19857), which has garnered 671 citations, reflecting its reliability in the field. This antibody is particularly effective for Western blotting (WB), immunocytochemistry (ICC), and immunoprecipitation (IP). In addition, we offer the recombinant antibody, Anti-Oct4 antibody [EPR17929] - ChIP Grade (ab181557). This product is validated for a broader range of applications, including WB, ICC, immunohistochemistry (IHC), flow cytometry (FC), chromatin immunoprecipitation (ChIP), and IP, making it a versatile choice for various experimental needs. With 185 citations, it is also gaining recognition among researchers. Both antibodies provide reliable options for studying POU5F1, catering to different experimental requirements. The Human/Mouse Oct4 ELISA Kit (ab235653) is an excellent option for researchers looking to measure POU5F1 in their samples.
Abcam Product Citation Summary
The data indicates a significant focus on the role of POU5F1 (also known as Oct4) in various cancer contexts, particularly in human and mouse models. The use of Abcam antibodies in studies related to pluripotency, stem cell characteristics, and cancer stem cell properties highlights the importance of POU5F1 in both developmental biology and oncology. The antibodies have been applied in multiple techniques, predominantly Western blotting, to explore the expression and function of POU5F1 in different cell types and conditions.
Abcam Product Citation Table
Developmental stage
Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
Domain
The POU-specific domain mediates interaction with PKM.
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Function
The protein expressed by the gene POU5F1 is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and forms a trimeric complex with SOX2 or SOX15 on DNA. It controls the expression of several genes involved in embryonic development, including YES1, FGF4, UTF1, and ZFP206. This protein is critical for early embryogenesis and for maintaining embryonic stem cell pluripotency. This supplementary information is collated from multiple sources and compiled automatically.
Post-translational modifications
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.
Sequence Similarities
Belongs to the POU transcription factor family. Class-5 subfamily.
Tissue Specificity
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Cellular localization
- Cytoplasm
- Nucleus
- Expressed in a diffuse and slightly punctuate pattern. Colocalizes with MAPK8 and MAPK9 in the nucleus.
Alternative names
OCT3, OCT4, OTF3, POU5F1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, Oct-3, Oct-4, OTF-3
Database links
swissprot:Q01860 omim:164177 entrezGene:5460
Other research areas
- Oncology