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PPARA

GeneName

PPARA

Summary

PPARA, also known as PPAR or peroxisome proliferator activated receptor alpha, is a 52 kDa nuclear receptor that functions as a transcription factor regulating gene expression in response to fatty acids and other ligands. It is primarily expressed in tissues involved in lipid metabolism, such as the liver, adipose tissue, and muscle. PPARA plays a critical role in various biological processes including fatty acid oxidation, glucose homeostasis, and inflammation. It binds to specific DNA sequences to modulate the transcription of target genes, influencing metabolic pathways and cellular responses to nutritional signals.

Importance

PPARA is relevant to: - Metabolic disorders, including obesity and type 2 diabetes, through its regulation of fatty acid and glucose metabolism. - Cardiovascular health, as it influences lipid profiles and inflammatory responses associated with atherosclerosis. - The circadian regulation of metabolism, impacting energy homeostasis and sleep patterns. - Drug development, particularly in the context of PPAR agonists used for treating dyslipidaemia and insulin resistance.

Top Products

For researchers investigating PPARA, we recommend two excellent primary antibodies. The first is the well-cited polyclonal antibody, Anti-PPAR alpha antibody (ab126285), which has garnered 36 citations, reflecting its reliability in Western blotting (WB). Additionally, we offer the recombinant antibody, Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865). This recombinant product is also validated for WB, providing the added benefit of batch-to-batch consistency that is often essential in research. Both antibodies are outstanding choices for those studying PPARA.

Abcam Product Citation Summary

The data indicates that PPARA is being studied primarily in mouse models, particularly in heart and liver tissues. The use of Western blotting suggests a focus on protein expression levels in these contexts, which may be linked to validating RNA-Sequencing results and understanding metabolic processes.

Abcam Product Citation Table

Product Code
Species
Application
Study Context
PMID
ab126285
Mouse
WB
Heart tissue
32313136
ab126285
Mouse
WB
39086681
ab61182
Mouse
WB
Liver tissues
32377219

Function

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as a transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.

Post-translational modifications

Ubiquitinated by E3 ubiquitin-protein ligase HUWE1; leading to proteasomal degradation.

Phosphorylated.

Sequence Similarities

Belongs to the nuclear hormone receptor family. NR1 subfamily.

Tissue Specificity

Skeletal muscle, liver, heart and kidney. Expressed in monocytes (PubMed:28167758).

Cellular localization

Alternative names

NR1C1, PPAR, PPARA, Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1

swissprot:Q07869 omim:170998 entrezGene:5465