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PPP1R12A phospho T853

Developmental stage

Induced by 2-fold during pregnancy, including in abdominus rectus muscle.

Domain

Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.

The KVKF motif mediates interaction with PPP1CB.

Function

Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.

Involvement in disease

Genitourinary and/or brain malformation syndrome

GUBS

An autosomal dominant syndrome characterized by multiple congenital anomalies including urogenital malformations and brain abnormalities ranging from agenesis of the corpus callosum to anencephaly.

None

The disease is caused by variants affecting the gene represented in this entry.

Post-translational modifications

Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. Phosphorylation at Ser-445, Ser-472 and Ser-910 by NUAK1 promotes interaction with 14-3-3, leading to inhibit interaction with myosin light chain MLC2, preventing dephosphorylation of MLC2. May be phosphorylated at Thr-696 by DMPK; may inhibit the myosin phosphatase activity. Phosphorylated at Ser-473 by CDK1 during mitosis, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates PPP1R12A.

Tissue specificity

Expressed in striated muscles, specifically in type 2a fibers (at protein level).

Cellular localization

  • Cytoplasm
  • Cytoplasm
  • Cytoskeleton
  • Stress fiber
  • Also along actomyosin filaments.

Alternative names

MBS, MYPT1, PPP1R12A, Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, Myosin phosphatase target subunit 1

Target type

Proteins

Primary research area

Oncology

Molecular weight

115281Da

We found 1 product in 1 category

Primary Antibodies

Application

Reactive species

Search our catalogue for 'PPP1R12A phospho T853' (1)

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