PTBP1
GeneName
PTBP1
Summary
PTBP1, also known as polypyrimidine tract binding protein 1 or PTB, is a 60 kDa RNA-binding protein that plays a pivotal role in the regulation of pre-mRNA splicing and mRNA processing. It is expressed in various cellular compartments, including the nucleus, nucleoplasm, nucleolus, and also found in extracellular exosomes and membranes. PTBP1 binds to mRNA and poly-pyrimidine tracts, influencing alternative splicing events and thereby regulating gene expression. Its involvement in neurogenesis and muscle cell differentiation highlights its importance in developmental processes.
Importance
PTBP1 is relevant to: - Understanding the mechanisms of mRNA splicing and processing, which are fundamental to gene regulation - Investigating its role in neurogenesis and muscle differentiation, providing insights into developmental biology - Exploring its function in IRES-dependent viral translational initiation, which has implications for virology - Studying its influence on calcineurin-NFAT signalling, relevant to immune responses and cellular differentiation - Its potential involvement in diseases associated with splicing dysregulation, such as certain cancers and neurodegenerative disorders
Top Products
For researchers investigating PTBP1, we highly recommend the top-selling recombinant antibody, Anti-PTBP1 antibody [EPR9048(B)] (ab133734). This well-cited antibody has garnered 41 citations, reflecting its reliability and trust within the research community. It has been validated in knockout models and is suitable for a variety of applications, including Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), and flow cytometry (FC). This versatility makes it an excellent choice for those seeking consistent and effective detection of PTBP1 in their studies.
Abcam Product Citation Summary
The data indicates that PTBP1 is being studied in the context of myodifferentiation of C2C12 myoblasts, highlighting its role in splicing regulation. Additionally, PTBP1 is also being investigated in human samples using immunocytochemistry and immunofluorescence techniques.
Abcam Product Citation Table
Function
Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10 (PubMed:15009664). Binds to polypyrimidine-rich controlling element (PCE) of CFTR and promotes exon skipping of CFTR exon 9, thereby antagonizing TIA1 and its role in exon inclusion of CFTR exon 9 (PubMed:14966131). Plays a role in the splicing of pyruvate kinase PKM by binding repressively to a polypyrimidine tract flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808). In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:21518806).
Cellular localization
- Nucleus
Alternative names
PTB, PTBP1, Polypyrimidine tract-binding protein 1, 57 kDa RNA-binding protein PPTB-1, Heterogeneous nuclear ribonucleoprotein I, hnRNP I