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RAB3A_HUMAN phospho T86

Function

Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane (By similarity). Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane (By similarity). Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed (By similarity). Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells (By similarity). Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9 (PubMed:27325790). Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1 (PubMed:22248876, PubMed:30599141). Plays also a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT (By similarity). Interacts with MADD (via uDENN domain); the GTP-bound form is preferred for interaction (By similarity).

Post-translational modifications

Phosphorylation of Thr-86 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Tissue specificity

Specifically expressed in brain.

Cellular localization

  • Cytoplasm
  • Cytosol
  • Lysosome
  • Cytoplasmic vesicle
  • Secretory vesicle
  • Cell projection
  • Axon
  • Cell membrane
  • Lipid-anchor
  • Cytoplasmic side
  • Cycles between a vesicle-associated GTP-bound form and a cytosolic GDP-bound form.

Alternative names

  • Ras-related protein Rab-3A
  • RAB3A

Target type

Proteins

Molecular weight

24984Da