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RARB

GeneName

RARB

Summary

RARB, also known as HAP or RAR beta, is a 50 kDa nuclear receptor that plays a pivotal role in regulating gene expression in response to retinoic acid. It is predominantly expressed in the nucleus and cytoplasm of various tissues, where it functions as a DNA-binding transcription factor. RARB is involved in several biological processes, including cell differentiation, neurogenesis, and the regulation of apoptosis. Its activity is mediated through binding to specific DNA sequences in the regulatory regions of target genes, influencing processes such as embryonic development and stem cell proliferation.

Importance

RARB is relevant to: - Developmental biology, particularly in the context of embryonic morphogenesis and organ development - Cancer research, due to its role in regulating cell differentiation and apoptosis - Neurological studies, as it influences neurogenesis and neural precursor cell proliferation - Stem cell research, given its involvement in stem cell proliferation and differentiation pathways

Top Products

For researchers investigating RARB, we recommend two excellent primary antibodies that offer reliable performance across various applications. The first is the well-cited polyclonal antibody, Anti-Retinoic Acid Receptor beta antibody (ab53161), which has garnered 25 citations, reflecting its trustworthiness in the field. This antibody is suitable for Western blotting (WB), immunohistochemistry (IHC), and immunocytochemistry (ICC), making it a versatile choice for your research needs.Additionally, we offer the recombinant antibody, Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701). This product has been validated for the same applications—WB, IHC, and ICC—and has 6 citations, indicating its growing recognition among researchers. The recombinant nature of this antibody ensures batch-to-batch consistency, providing you with reliable results in your studies of RARB.

Abcam Product Citation Summary

The data indicates that RARB antibodies have been employed in various studies focusing on human and mouse models. The use of immunohistochemistry in human lung tissue and cell lines highlights the relevance of RARB in conditions such as TSC mutant diseases and cellular migration. Additionally, Western blotting in mouse striatal protein samples suggests a role for RARB in neurological contexts.

Abcam Product Citation Table

Product Code
Species
Application
Study Context
PMID
ab124701
Human
IHC
LAM cell lines
34178631
ab124701
Human
IHC
Cellular migration and proliferation
34178631
ab124701
Human
IHC
Lung tissue
34178631
ab53161
Mouse
WB
Striatal protein samples
32286473
ab53161
Mouse
WB
Striatal protein samples
32286473

Domain

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

The DNA-binding nuclear receptor domain and the NR LBD domain are required for binding of the RARB/RXRA heterodimer to both DR1 and DR5 DNA elements.

Function

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors (PubMed:12554770). The RXRA/RARB heterodimer can act as a repressor on the DR1 element and as an activator on the DR5 element (PubMed:29021580). In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity).

Involvement in disease

Microphthalmia, syndromic, 12

MCOPS12

A form of microphthalmia, a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS12 patients manifest variable features, including diaphragmatic hernia, pulmonary hypoplasia, and cardiac abnormalities.

None

The disease is caused by variants affecting the gene represented in this entry.

Sequence Similarities

Belongs to the nuclear hormone receptor family. NR1 subfamily.

Tissue Specificity

Expressed in aortic endothelial cells (at protein level).

Cellular localization

Alternative names

HAP, NR1B2, RARB, Retinoic acid receptor beta, RAR-beta, HBV-activated protein, Nuclear receptor subfamily 1 group B member 2, RAR-epsilon

swissprot:P10826 omim:180220 entrezGene:5915