Ras-related protein Rap-2a

Domain

The effector domain mediates the interaction with RUNDC3A.

Function

Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.

Post-translational modifications

Ubiquitinated; undergoes 'Lys-63' monoubiquitination and diubiquitination by NEDD4. Multiple lysine residues are probably modified. Ubiquitination requires TNIK, prevents interaction with effectors and inactivates RAP2A.

Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK.

(Microbial infection) Glucosylated at Thr-35 by C.difficile toxin TcdA in the colonic epithelium, and by P.sordellii toxin TcsL in the vascular endothelium.

Sequence Similarities

Belongs to the small GTPase superfamily. Ras family.

Cellular localization

• Recycling endosome membrane
• Lipid-anchor
• Cytoplasmic side
• Midbody
• May also localize to the Golgi (PubMed:7962206) and the gelatinase-containing granules of neutrophils (PubMed:8391995). Colocalizes with RASGEF1B to midbody at telophase (PubMed:23894443).

Alternative names

Ras-related protein Rap-2a, RbBP-30, RAP2A

Database links

swissprot:P10114 swissprot:Q9Y3L5 omim:179540 entrezGene:57826 entrezGene:5912 omim:179541 swissprot:P61225 entrezGene:5911