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RASA1

GeneName

RASA1

Summary

RASA1, also known as GAP, GTPase activating protein, or RasGAP, is a 116 kDa protein that functions primarily in the cytoplasm and at the plasma membrane. It is involved in the negative regulation of RAS proteins, which are critical for various signalling pathways. RASA1 plays a role in blood vessel morphogenesis and vasculogenesis, and it is implicated in intracellular signal transduction processes. Additionally, it has functions related to cell adhesion and the regulation of actin filament polymerization, influencing cell shape and behaviour during mitotic cytokinesis.

Importance

RASA1 is relevant to: - Vascular development and pathology, as it regulates processes essential for blood vessel formation and maintenance - Cancer research, due to its role in RAS signalling pathways, which are frequently mutated in various cancers - Neurobiology, given its involvement in the regulation of neuronal apoptosis and cell adhesion, impacting neural development and neurodegenerative diseases - Cell biology, through its functions in actin dynamics and cell morphology, which are critical for cell migration and tissue organisation

Top Products

For researchers investigating RASA1, we highly recommend the top-selling recombinant antibody, Anti-RASA1 antibody [EP536Y] (ab40677). This antibody has been validated for use in Western blotting (WB) and immunohistochemistry (IHC), making it a versatile tool for various experimental setups. With 12 citations, it demonstrates a solid presence in the research community, reflecting its reliability and effectiveness in detecting RASA1. This recombinant antibody offers the added benefit of batch-to-batch consistency, ensuring dependable results in your studies.

Abcam Product Citation Summary

The data indicates that RASA1 is being studied in various contexts, particularly in relation to microRNA manipulation and its role in neovascularization. The use of RASA1 antibodies in human endothelial cells and hepatocellular carcinoma tissues highlights its potential significance in angiogenesis and ischaemia-related research.

Abcam Product Citation Table

Product Code
Species
Application
Study Context
PMID
ab2922
Human
WB
Ischaemia
25945589
ab2922
Human
Neovascularization assay
25945589
ab40677
Human
WB
Hepatocellular carcinoma
26126858
ab40677
Human
WB, IHC
Hypoxia conditions
26126858

Function

GTPase-activating protein (GAP) that stimulates the intrinsic GTPase activity of Ras proteins, such as NRAS, facilitating their transition from the active GTP-bound state to the inactive GDP-bound state, thereby terminating Ras signaling.

Involvement in disease

Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.

Capillary malformation-arteriovenous malformation 1

CMAVM1

A disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome. CMAVM1 inheritance is autosomal dominant.

None

The disease is caused by variants affecting the gene represented in this entry.

Post-translational modifications

The N-terminus is blocked.

Phosphorylated by SRC and LCK. The phosphorylation SRC inhibits its ability to stimulate the Ras-GTPase activity, whereas phosphorylation by LCK does not display any effect on stimulation activity.

Tissue Specificity

In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level).

Cellular localization

Alternative names

GAP, RASA, RASA1, Ras GTPase-activating protein 1, GTPase-activating protein, RasGAP, Ras p21 protein activator, p120GAP

swissprot:P20936 entrezGene:5921 omim:139150