RECQL5

The alpha-helix immediately C-terminal to the Zn(2+)-binding site (residues 438-453, characterized in C-terminally truncated contructs) is required for helicase activity, is positively charged, and may play a role in DNA-binding and choice of substrates (PubMed:28100692).

DNA helicase that plays an important role in DNA replication, transcription and repair (PubMed:20643585, PubMed:22973052, PubMed:28100692). Probably unwinds DNA in a 3'-5' direction (Probable) (PubMed:28100692). Binds to the RNA polymerase II subunit POLR2A during transcription elongation and suppresses transcription-associated genomic instability (PubMed:20231364). Associates also with POLR1A and enforces the stability of ribosomal DNA arrays (PubMed:27502483). Plays an important role in mitotic chromosome separation after cross-over events and cell cycle progress (PubMed:22013166). Mechanistically, removes RAD51 filaments protecting stalled replication forks at common fragile sites and stimulates MUS81-EME1 endonuclease leading to mitotic DNA synthesis (PubMed:28575661). Required for efficient DNA repair, including repair of inter-strand cross-links (PubMed:23715498). Stimulates DNA decatenation mediated by TOP2A. Prevents sister chromatid exchange and homologous recombination. A core helicase fragment (residues 11-609) binds preferentially to splayed duplex, looped and ssDNA (PubMed:28100692).

Phosphorylated by CDK1 at Ser-727; this phosphorylation is required for RECQL5-mediated disruption of RAD51 filaments on stalled replication forks.

Belongs to the helicase family. RecQ subfamily.

Ubiquitous.

• Isoform Beta
• Nucleus
• Nucleoplasm
• Nucleus
• Recruited to sites of DNA damage, such as single-strand breaks and inter-strand cross-links, and at stalled replication forks. Re-localizes from the nucleolus into the nucleus after replicative stress and significantly associates with WRN during S-phase (PubMed:23180761).
• Isoform Alpha
• Cytoplasm
• Isoform Gamma
• Cytoplasm

RECQ5, RECQL5, ATP-dependent DNA helicase Q5, DNA 3'-5' helicase RecQ5, RecQ protein-like 5, RecQ5

• entrezGene:9400
• omim:603781
• swissprot:O94762

Proteins

108858Da