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ROCK2 phospho S1366

Domain

An interaction between Thr-414 and Asp-48 is essential for kinase activity and dimerization.

Function

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

Post-translational modifications

Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity.

Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Tissue specificity

Expressed in the brain (at protein level).

Cellular localization

  • Cytoplasm
  • Cell membrane
  • Peripheral membrane protein
  • Nucleus
  • Cytoplasm
  • Cytoskeleton
  • Microtubule organizing center
  • Centrosome
  • Cytoplasmic, and associated with actin microfilaments and the plasma membrane.

Alternative names

  • Rho-associated protein kinase 2
  • Rho kinase 2
  • p164 ROCK-2
  • ROCK-II
  • ROCK2
  • KIAA0619

Target type

Proteins

Primary research area

Oncology

Molecular weight

160900Da