RRM2
GeneName
RRM2
Summary
RRM2, also known as RR2, hRRM2, or RR-2, is a 45 kDa protein that plays a pivotal role in the biosynthesis of deoxyribonucleotides, which are essential for DNA synthesis and repair. It is primarily located in the cytosol and nucleoplasm, and is a component of the ribonucleoside-diphosphate reductase complex. RRM2 exhibits ferric iron binding and has protein homodimerization activity, as well as ribonucleoside-diphosphate reductase activity, where it acts as a thioredoxin disulfide acceptor. This protein is involved in various biological processes including the positive regulation of the G1/S transition in the mitotic cell cycle and the metabolic processes of ribonucleoside diphosphates, contributing to cellular proliferation and development.
Importance
RRM2 is relevant to: - Cancer research, as it is essential for DNA synthesis and cell proliferation, making it a potential target for therapeutic intervention. - Stem cell biology and developmental studies due to its role in blastocyst development and cellular differentiation. - Understanding cell cycle regulation, particularly in the context of diseases characterised by uncontrolled cell growth. - Investigating metabolic disorders related to nucleotide metabolism, which can have implications in various genetic conditions.
Top Products
For researchers investigating RRM2, we recommend two excellent primary antibodies. The first is the well-cited Anti-RRM2 antibody [1E1] (ab57653), which has garnered 44 citations and is highly regarded for its performance in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), and flow cytometry (FC). This antibody is a reliable choice for those looking to study RRM2 in various applications. Additionally, we offer the recombinant antibody, Anti-RRM2 antibody [EPR11820] (ab172476). This product has been validated in multiple applications, including WB, IHC, ICC, and immunoprecipitation (IP), making it a versatile option for researchers who require the consistency that recombinant antibodies provide. With 29 citations, it is also gaining traction in the research community. Together, these antibodies provide robust tools for studying RRM2 effectively.
Abcam Product Citation Summary
The data indicates that the RRM2 target is being investigated in the context of glioblastoma, particularly focusing on the effects of BRCA1 loss and regulation. Both western blotting and immunohistochemistry applications were employed to study RRM2 expression in human GBM cells.
Abcam Product Citation Table
Function
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
Post-translational modifications
Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling. Phosphorylated on Thr-33 by CDK1 and CDK2; predominantly in G2 and M phase (PubMed:22632967).
Ubiquitinated by the SCF(CCNF) E3 ubiquitin-protein ligase complex; leading to its degradation by the proteasome.
Sequence Similarities
Belongs to the ribonucleoside diphosphate reductase small chain family.
Cellular localization
- Cytoplasm
- Nucleus
- Localized to the cytoplasm in S phase cells. May localize to the nucleus in G2 phase cells.
Alternative names
RR2, RRM2, Ribonucleoside-diphosphate reductase subunit M2, Ribonucleotide reductase small chain, Ribonucleotide reductase small subunit
Database links
swissprot:P31350 entrezGene:50484 omim:180390 omim:604712 swissprot:Q7LG56 entrezGene:6241