RUFY1
Domain
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions. The 2 coiled coil domains mediate the interaction with the dynein-dynactin complex, the RUN domain is also involved in the interaction (PubMed:36282215). The coiled coil domains also mediate self-association (By similarity).
Function
Activating adapter involved in cargo sorting from early/recycling endosomes. Regulates retrieval of proteins from endosomes to the trans-Golgi network through interaction with the dynein-dynactin complex (PubMed:36282215). Dual effector of RAB4B and RAB14, mediates a cooperative interaction allowing endosomal tethering and fusion (PubMed:20534812). Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking (PubMed:14617813). In oocytes, self-assembles to form a protein matrix which hold together endolysosomes, autophagosomes and proteasomes and generate non-membrane-bound compartments called endo-lysosomal vesicular assemblies (ELVAs). In immature oocytes, ELVAs sequester ubiquitinated protein aggregates and degrade them upon oocyte maturation (By similarity).
Post-translational modifications
Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting.
Tissue Specificity
Broadly expressed, with highest levels in lung, testis, kidney and brain.
Cellular localization
- Early endosome membrane
- Peripheral membrane protein
- In oocytes, localizes in non-membrane-bound compartments called endo-lysosomal vesicular assemblies (ELVAs).
Alternative names
RABIP4, ZFYVE12, RUFY1, RUN and FYVE domain-containing protein 1, FYVE-finger protein EIP1, La-binding protein 1, Rab4-interacting protein, Zinc finger FYVE domain-containing protein 12