Serum response factor
GeneName
SRF
Summary
SRF, or serum response factor, is a 52 kDa transcription factor that plays a crucial role in regulating gene expression in response to various stimuli. It is predominantly located in the nucleus, but also associates with chromatin, cytoplasm, and nucleoplasm. SRF binds to serum response elements in the promoter regions of target genes, facilitating RNA polymerase II-mediated transcription. It is involved in numerous biological processes, including actin cytoskeleton organisation, angiogenesis, cardiac muscle cell differentiation, and neuron development. SRF is essential for cellular responses to growth factors and hormones, influencing cell migration, differentiation, and survival.
Importance
SRF is relevant to: - Cardiovascular research due to its role in cardiac muscle cell differentiation and heart development - Cancer biology, as it regulates processes such as cell migration and angiogenesis, which are critical for tumour growth and metastasis - Neurobiology, given its involvement in neuron development and long-term memory - Wound healing and tissue regeneration through its regulation of angiogenesis and cell adhesion
Top Products
For researchers investigating Serum Response Factor (SRF), we recommend two excellent primary antibodies. The first is the well-cited polyclonal antibody, Anti-Serum Response Factor SRF antibody (ab53147), which has garnered 17 citations and is highly regarded for its performance in Western blotting (WB) and immunohistochemistry (IHC). This antibody is a trusted choice for those looking to study SRF in various contexts. Additionally, we offer the recombinant antibody, Anti-Serum Response Factor SRF antibody [2C5] (ab252868). This monoclonal antibody has been validated for use in WB, IHC, and immunoprecipitation (IP), making it a versatile option for researchers who require the consistency and reliability that recombinant antibodies provide. With 3 citations, it is gaining traction in the research community as a dependable tool for SRF detection.
Abcam Product Citation Summary
The data indicates that the SRF target is being investigated in the context of cancer cell migration, highlighting its potential role in this biological process. The use of both western blotting and immunohistochemistry suggests a thorough approach to understanding SRF expression in human fibroblasts.
Abcam Product Citation Table
Function
SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.
Post-translational modifications
Phosphorylated by PRKDC.
Cellular localization
- Nucleus
Alternative names
Serum response factor, SRF