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Function

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:12234937, PubMed:27720643, PubMed:32494006, PubMed:34822310). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed:32494006, PubMed:34822310). Within the 17S U2 SnRNP complex, SF3B1 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed:12234937). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). May also be involved in the assembly of the 'E' complex (PubMed:10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed:15146077, PubMed:33509932). Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92 (By similarity).

Post-translational modifications

Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis.

Citrullinated by PADI4.

Sequence similarities

Belongs to the SF3B1 family.

Cellular localization

  • Nucleus
  • Nucleus speckle
  • During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.

Alternative names

SAP155, SF3B1, Splicing factor 3B subunit 1, Pre-mRNA-splicing factor SF3b 155 kDa subunit, Spliceosome-associated protein 155, SF3b155, SAP 155

Target type

Proteins

Primary research area

Metabolism

Molecular weight

145830Da

We found 12 products in 1 category

Search our catalogue for 'SF3B1' (12)

Products

ab172634

Anti-SF3B1 antibody [EPR11986]

Recombinant
RabMAb

ab170854

Anti-SF3B1 antibody [EPR11987(B)]

Recombinant
RabMAb

ab202926

HRP Anti-SF3B1 antibody [EPR11986]

Recombinant
RabMAb

ab320195

APC Anti-SF3B1 antibody [EPR11986]

Recombinant
RabMAb

ab320194

PE Anti-SF3B1 antibody [EPR11986]

Recombinant
RabMAb