SIRT3
GeneName
SIRT3
Summary
SIRT3, also known as SIRT-3 or hSIRT3, is a 44 kDa mitochondrial protein that primarily resides in the mitochondrial matrix. It is a member of the sirtuin family of proteins and functions as an NAD+-dependent deacetylase, playing a critical role in regulating various metabolic pathways. SIRT3 is involved in the deacetylation of lysine residues on target proteins, influencing processes such as aerobic respiration and the cellular response to stress. Additionally, it participates in the regulation of reactive oxygen species and has roles in the positive regulation of insulin secretion and oxidative phosphorylation.
Importance
SIRT3 is relevant to: - Metabolic regulation and mitochondrial function, as it modulates key enzymes involved in energy production and oxidative stress response - Ageing and longevity research due to its involvement in cellular stress resistance and metabolic health - Cancer biology, where it may influence tumour metabolism and cell survival through its deacetylase activity - Cardiovascular health, as it plays a role in maintaining mitochondrial integrity and function in heart tissues
Top Products
For researchers investigating SIRT3, we highly recommend the top-selling recombinant antibody, Anti-SIRT3 antibody [EPR19755] (ab217319). This antibody has been validated for use in Western blotting (WB) and immunohistochemistry (IHC), making it a versatile tool for various applications in your research. With 35 citations, it is well-regarded in the scientific community, reflecting its reliability and effectiveness in SIRT3 detection. This recombinant antibody ensures batch-to-batch consistency, providing confidence in your experimental results. The Recombinant Human SIRT3 protein (Tagged) ELISA Kit (ab125586) is an excellent option for researchers looking to measure SIRT3 levels in their samples.
Abcam Product Citation Summary
The data indicates that the SIRT3 antibody (ab217319) has been consistently used in Western blotting to study its role in Alzheimer's disease using mouse models. This suggests a significant interest in understanding the implications of SIRT3 in the context of neurodegenerative disorders.
Abcam Product Citation Table
Function
NAD-dependent protein deacetylase (PubMed:12186850, PubMed:12374852, PubMed:16788062, PubMed:18680753, PubMed:18794531, PubMed:19535340, PubMed:23283301, PubMed:24121500, PubMed:24252090). Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed:12186850, PubMed:12374852, PubMed:16788062, PubMed:18680753, PubMed:18794531, PubMed:23283301, PubMed:24121500, PubMed:24252090). Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5PO (PubMed:16788062, PubMed:18680753, PubMed:19535340, PubMed:24121500, PubMed:24252090). Contributes to the regulation of the cellular energy metabolism (PubMed:24252090). Important for regulating tissue-specific ATP levels (PubMed:18794531). In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed:23283301). Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (By similarity). Regulates hepatic lipogenesis (By similarity). Uses NAD(+) substrate imported by SLC25A47, triggering downstream activation of PRKAA1/AMPK-alpha signaling cascade that ultimately downregulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance (By similarity). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by mediating delactylation of proteins, such as CCNE2 and 'Lys-16' of histone H4 (H4K16la) (PubMed:36896611, PubMed:37720100).
Post-translational modifications
Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
Sequence Similarities
Belongs to the sirtuin family. Class I subfamily.
Tissue Specificity
Widely expressed.
Cellular localization
- Mitochondrion matrix
Alternative names
SIR2L3, SIRT3, hSIRT3, NAD-dependent protein delactylase sirtuin-3, Regulatory protein SIR2 homolog 3, SIR2-like protein 3