SLC8A1
GeneName
SLC8A1
Summary
SLC8A1, also known as NCX1 or CNC, is a 109kDa transmembrane protein primarily expressed in cardiac and neuronal tissues. It functions as a calcium:sodium antiporter, facilitating the exchange of calcium ions and sodium ions across the plasma membrane. This protein is crucial for maintaining intracellular calcium homeostasis, particularly in cardiac muscle cells where it plays a vital role in muscle contraction and relaxation. SLC8A1 is localised to various cellular components, including the plasma membrane, axon, dendrite, and intercalated discs, and is involved in processes such as cardiac conduction and cellular responses to stimuli like caffeine and reactive oxygen species.
Importance
SLC8A1 is relevant to: - Cardiac health due to its role in regulating calcium ion transport, which is essential for heart muscle contraction and relaxation - Neurological function as it contributes to calcium homeostasis in neurons, influencing neuronal excitability and signalling - Understanding cardiac arrhythmias and heart failure, as dysregulation of calcium handling can lead to these conditions - Research into muscle physiology and pathophysiology, particularly in relation to muscle contraction mechanisms and responses to stress
Top Products
For researchers investigating SLC8A1, we recommend two primary antibodies that stand out for their performance and reliability. The first is the well-cited Anti-NCX1 antibody [C2C12] (ab2869), a monoclonal antibody that has garnered 34 citations, highlighting its trustworthiness in the field. This antibody is particularly effective for immunohistochemistry (IHC) and flow cytometry (FC), making it a solid choice for various experimental setups.In addition, we offer the recombinant antibody, Anti-NCX1 antibody [EPR12739] (ab177952), which has been validated for use in Western blotting (WB) and has received 21 citations. This recombinant product ensures batch-to-batch consistency, providing researchers with a dependable option for their studies on SLC8A1. Together, these antibodies provide a comprehensive toolkit for effective SLC8A1 research.
Abcam Product Citation Summary
The data indicates that SLC8A1 is being studied primarily in the context of brain injuries in mice, with multiple studies utilising the same antibody for detection. Additionally, there are studies involving human astrocytes and rat myocardium, suggesting a broader interest in the role of SLC8A1 in various biological contexts, including cardiac health and neurological conditions.
Abcam Product Citation Table
Domain
The cytoplasmic Calx-beta domains bind the regulatory Ca(2+). The first Calx-beta domain can bind up to four Ca(2+) ions. The second domain can bind another two Ca(2+) ions that are essential for calcium-regulated ion exchange.
Function
Mediates the exchange of one Ca(2+) ion against three to four Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes (PubMed:11241183, PubMed:1374913, PubMed:1476165). Contributes to Ca(2+) transport during excitation-contraction coupling in muscle (PubMed:11241183, PubMed:1374913, PubMed:1476165). In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum (PubMed:11241183, PubMed:1374913, PubMed:1476165). SLC8A1 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline (PubMed:11241183, PubMed:1374913, PubMed:1476165). Required for normal embryonic heart development and the onset of heart contractions (By similarity).
Sequence Similarities
Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC8 subfamily.
Tissue Specificity
Detected primarily in heart and at lower levels in brain (PubMed:1374913). Expressed in cardiac sarcolemma, brain, kidney, liver, pancreas, skeletal muscle, placenta and lung (PubMed:1476165).
Cellular localization
- Cell membrane
- Multi-pass membrane protein
Alternative names
CNC, NCX1, SLC8A1, Sodium/calcium exchanger 1, Na(+)/Ca(2+)-exchange protein 1, Solute carrier family 8 member 1