Snap25
Function
t-SNARE involved in the molecular regulation of neurotransmitter release (PubMed:8103915, PubMed:8243676). May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF (PubMed:16672379). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells (By similarity).
Post-translational modifications
Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type A (BoNT/A, botA) which hydrolyzes the 197-Gln-|-Arg-198 bond and inhibits neurotransmitter release (PubMed:8103915, PubMed:8243676).
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type E (BoNT/E) which hydrolyzes the 180-Arg-|-Ile-181 bond and inhibits neurotransmitter release (PubMed:8103915, PubMed:8243676).
Sequence Similarities
Belongs to the SNAP-25 family.
Tissue Specificity
Expressed in the outer nuclear layer of the retina (at protein level).
Cellular localization
- Cytoplasm
- Perinuclear region
- Cell membrane
- Lipid-anchor
- Synapse
- Synaptosome
- Photoreceptor inner segment
- Membrane association requires palmitoylation (PubMed:9349529). Expressed throughout cytoplasm, concentrating at the perinuclear region (PubMed:16672379). Colocalizes with KCNB1 at the cell membrane (By similarity). Colocalizes with PLCL1 at the cell membrane (By similarity).
Alternative names
Snap, Synaptosomal-associated protein 25, SNAP-25, Super protein, Synaptosomal-associated 25 kDa protein, SUP