SOCS3
GeneName
SOCS3
Summary
SOCS3, also known as SOCS-3, cis-3, or CIS3, is a 25 kDa protein that functions primarily as a negative regulator of cytokine signalling. It is expressed in various tissues and is localised to the cytoplasmic side of the plasma membrane and the cytosol. SOCS3 interacts with cytokine receptors, inhibiting their signalling pathways, particularly those mediated by the JAK-STAT pathway. It plays a role in several biological processes, including the cellular response to interleukin-17 and leukemia inhibitory factor, and is involved in the regulation of apoptosis and inflammation. Additionally, SOCS3 contributes to processes such as placenta blood vessel development and T-helper 17 cell lineage commitment.
Importance
SOCS3 is relevant to: - The regulation of cytokine signalling, which is essential for immune responses and inflammation - The modulation of T-helper cell differentiation, particularly in the context of autoimmune diseases - The potential role in cancer biology, where it may influence tumour progression through its effects on cell signalling - Metabolic disorders, as it regulates insulin receptor signalling and may impact insulin sensitivity - Developmental processes, including morphogenesis of the labyrinthine layer in the placenta
Top Products
For researchers investigating SOCS3, we recommend two excellent primary antibodies. The first is the well-cited polyclonal antibody, Anti-SOCS3 antibody (ab16030), which has garnered 161 citations, underscoring its reliability in Western blotting (WB) and immunocytochemistry (ICC). This antibody is a trusted choice for those looking to study SOCS3 in detail. Additionally, we offer the recombinant antibody, Anti-SOCS3 antibody [EPR24090-74] (ab280884), which is validated for use in Western blotting (WB), immunohistochemistry (IHC), and immunoprecipitation (IP). With 19 citations, this recombinant product provides the batch-to-batch consistency that many researchers seek. Together, these antibodies offer versatile options for studying SOCS3 effectively.
Abcam Product Citation Summary
The data indicates that SOCS3 is being studied in various contexts, particularly in relation to cancer, macrophage behaviour, and pulmonary fibrosis. The use of Abcam's antibody ab16030 in both human and mouse models highlights its relevance in understanding SOCS3's role in these biological processes.
Abcam Product Citation Table
Domain
The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
Function
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including IL6ST/gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity and regulates IL6 signaling. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:15601820).
Involvement in disease
There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
Pathway
Protein modification; protein ubiquitination.
Post-translational modifications
Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
Tissue Specificity
Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
Alternative names
CIS3, SSI3, SOCS3, Suppressor of cytokine signaling 3, SOCS-3, Cytokine-inducible SH2 protein 3, STAT-induced STAT inhibitor 3, CIS-3, SSI-3