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SOX2

GeneName

SOX2

Summary

SOX2, also known as Sox-2 or transcription factor Sox2, is a 34kDa transcription factor that is predominantly expressed in pluripotent stem cells and various developing tissues. It is localised within the nucleus and cytoplasm, where it plays a vital role in regulating gene expression by binding to specific DNA sequences. SOX2 is crucial for maintaining the self-renewal and pluripotency of stem cells, and it is involved in several developmental processes including brain, eye, and pituitary gland development. Additionally, SOX2 influences cell fate decisions and differentiation through its regulatory functions in chromatin organisation and transcriptional activation.

Importance

SOX2 is relevant to: - Stem cell biology, as it is essential for the maintenance of pluripotency and self-renewal in embryonic and induced pluripotent stem cells. - Developmental biology, due to its involvement in the differentiation processes of various tissues, including the nervous system and endocrine organs. - Cancer research, as aberrant expression of SOX2 has been linked to the maintenance of cancer stem cells and tumour progression in various malignancies. - Regenerative medicine, where understanding SOX2 function can inform strategies for tissue regeneration and repair.

Top Products

For researchers investigating SOX2, we recommend two excellent primary antibodies. The first is the highly regarded polyclonal antibody, Anti-SOX2 antibody (ab97959), which has garnered 917 citations, reflecting its strong reputation in the field. This antibody is well-suited for immunohistochemistry (IHC), western blotting (WB), and immunocytochemistry (ICC), making it a versatile choice for various applications.In addition, we offer the recombinant antibody, Anti-SOX2 antibody [EPR3131] (ab92494). This monoclonal antibody has been validated for use in IHC, WB, ICC, and ELISA, providing researchers with the batch-to-batch consistency that recombinant antibodies are known for. With 328 citations, it is also gaining traction in the research community. Together, these antibodies provide robust options for studying SOX2 effectively. The Anti-SOX2 antibody [SOX2/1792] ELISA Kit (ab218521) is an excellent option for researchers looking to measure SOX2 levels in their samples.

Abcam Product Citation Summary

The data indicates a significant focus on the role of SOX2 in various stem cell contexts, particularly in human and mouse models. Studies highlight its involvement in cancer stem cell characteristics, pluripotency, and differentiation across multiple cancer types, including breast, prostate, and liver cancers. The use of SOX2 antibodies in both Western blotting and immunohistochemistry suggests its critical role in understanding stem cell biology and cancer progression.

Abcam Product Citation Table

Product Code
Species
Application
Study Context
PMID
ab137385
Human
WB
Breast cancer cell lines
30482236
ab171380
Human
WB, IHC
Glioblastoma stem cells
30250190
ab171380
Human
WB, IHC
Glycolytic reprogramming and GSC phenotypic transition
30250190
ab171380
Human
WB, IHC
Effects of VDAC2 overexpression on glioma stem cells
30250190
ab79351
Human
IF
Embryonic stem cell-derived neural progenitor cells
25799239
ab92494
Human
WB
TNBC cells
30324719
ab92494
Human
WB
HCC PDOs
31992334
ab92494
Human
WB
PMSCs under low oxygen tension
29138637
ab92494
Human
WB
Prostate cancer stem cells
35096586
ab97959
Human
WB
Liver cancer cells
29722127
ab97959
Human
WB
Huh7 cells
25943891
ab97959
Mouse
IHC
Brain tissue after tMCAO
33391532
ab97959
Mouse
WB
Induced neural stem cells
26830441

Domain

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Function

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:18035408). Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity).

Involvement in disease

Microphthalmia, syndromic, 3

MCOPS3

A disease characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula. Microphthalmia is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities.

None

The disease is caused by variants affecting the gene represented in this entry.

Post-translational modifications

Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.

Methylation at Lys-42 and Lys-117 is necessary for the regulation of SOX2 proteasomal degradation.

Ubiquitinated by WWP2, leading to proteasomal degradation.

Cellular localization

Alternative names

Transcription factor SOX-2, SOX2

swissprot:P48431 entrezGene:6657 omim:184429

Other research areas