SP7
GeneName
SP7
Summary
SP7, also known as MP2 or Osx, is a transcription factor that plays a vital role in osteoblast differentiation and bone formation. It is expressed in various tissues, particularly in osteoblasts, and is localised to the nucleus, chromatin, and cytoplasm. SP7 binds to DNA and regulates gene expression through its activity as a transcription activator specific to RNA polymerase II. Additionally, it is involved in cellular responses to zinc ion levels and has a role in hematopoietic stem cell differentiation. The protein also interacts with DEAD/H-box RNA helicases and binds zinc ions, which may influence its function in transcriptional regulation.
Importance
SP7 is relevant to: - Osteogenesis and bone health due to its essential role in osteoblast differentiation and mineralisation processes - Stem cell biology, particularly in the context of hematopoietic stem cell differentiation and maintenance - Regulation of gene expression in response to environmental cues, such as zinc ion availability - Potential therapeutic targets in conditions related to bone density and mineralisation disorders
Top Products
For researchers investigating SP7, we highly recommend the top-selling recombinant antibody, Anti-Sp7 / Osterix antibody [EPR21034] (ab209484). This antibody has been validated for use in several applications, including Western blotting (WB), immunohistochemistry (IHC), and immunoprecipitation (IP), making it a versatile tool for your research needs. With 246 citations, it is well-regarded in the scientific community, reflecting its reliability and effectiveness in SP7 studies. This recombinant antibody ensures batch-to-batch consistency, providing confidence in your experimental results.
Abcam Product Citation Summary
The data indicates that the SP7 antibody (ab209484) has been effectively used in Western blotting to study its role in bone formation and osteogenic differentiation in both mouse and rat models. The studies highlight the importance of SP7 in understanding bone-related processes, particularly in the context of gossypol's effects and the differentiation of bone marrow mesenchymal stem cells (BMMSCs).
Abcam Product Citation Table
Domain
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Function
Transcriptional activator essential for osteoblast differentiation (PubMed:23457570). Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences (By similarity).
Involvement in disease
Osteogenesis imperfecta 12
OI12
A form of osteogenesis imperfecta, a disorder of bone formation characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. Disease severity ranges from very mild forms without fractures to intrauterine fractures and perinatal lethality. Extraskeletal manifestations, which affect a variable number of patients, are dentinogenesis imperfecta, hearing loss, and blue sclerae. OI12 is an autosomal recessive form characterized by recurrent fractures, mild bone deformations, delayed teeth eruption, no dentinogenesis imperfecta, normal hearing, and white sclerae.
None
The disease is caused by variants affecting the gene represented in this entry.
Post-translational modifications
Ubiquitination at leads to proteasomal degradation. SP7 is a short-live protein with an endogenous half-life of approximately 12 hours.
Propionylated. Depropionylation at Lys-371 by SIRT7 activates transcription factor activity and positively regulates bone formation by osteoblasts.
Sequence Similarities
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Tissue Specificity
Restricted to bone-derived cell.
Cellular localization
- Nucleus
Alternative names
OSX, SP7, Transcription factor Sp7, Zinc finger protein osterix