SPDL1
Function
Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment (PubMed:17576797, PubMed:19468067). Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:25035494). Plays a role in cell migration (PubMed:30258100).
Post-translational modifications
Monoubiquitinated with'Lys-48' linkage (PubMed:30258100). Deubiquitinated by USP45 (PubMed:30258100).
Sequence Similarities
Belongs to the Spindly family.
Cellular localization
- Cytoplasm
- Cytoskeleton
- Microtubule organizing center
- Centrosome
- Chromosome
- Centromere
- Kinetochore
- Nucleus
- Cytoplasm
- Cytoskeleton
- Spindle pole
- Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.
Alternative names
CCDC99, SPDL1, Protein Spindly, hSpindly, Arsenite-related gene 1 protein, Coiled-coil domain-containing protein 99, Rhabdomyosarcoma antigen MU-RMS-40.4A, Spindle apparatus coiled-coil domain-containing protein 1