TIAM2
Domain
The PH 1 domain and amino acids 621-782 (a region called TSS; otherwise known as CC-Ex) are necessary for membrane localization. The PH 1 and TSS domains are necessary for Rac1 activity. The PH 2 domain is engaged in the enhancement of the catalytic activity of the adjacent DH domain. The PH 1, TSS and DH domains are necessary to induce neurite-like structure (By similarity).
Function
Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane (By similarity). Activates specifically RAC1, but not CDC42 and RHOA.
Post-translational modifications
Phosphorylated on serine and threonine residues. Phosphorylated on Thr-1648 by Rho-kinase. Its phosphorylation by Rho-kinase inhibits its guanine nucleotide exchange activity, its interaction with MAP1A, MAP1B, PARP1 and YWHAE and reduces its ability to promote neurite growth (By similarity).
Sequence Similarities
Belongs to the TIAM family.
Tissue Specificity
Expressed in the occipital, frontal and temporal lobes, cerebellum, putamen and testis.
Cellular localization
- Cytoplasm
- Cell projection
- Lamellipodium
- Cell projection
- Filopodium
- Cell projection
- Growth cone
- Cell projection
- Neuron projection
- Perikaryon
Alternative names
KIAA2016, STEF, TIAM2, Rho guanine nucleotide exchange factor TIAM2, SIF and TIAM1-like exchange factor, T-lymphoma invasion and metastasis-inducing protein 2, TIAM-2