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TIMP1

GeneName

TIMP1

Summary

TIMP1, also known as EPA or TIMP-1, is a 23 kDa secreted protein that plays a vital role in the regulation of matrix metalloproteinases (MMPs) by inhibiting their activity. It is primarily expressed in the extracellular matrix and is found in various tissues, including the basement membrane and extracellular space. TIMP1 is involved in several biological processes such as cartilage development, wound healing, and the regulation of apoptotic processes. Its functions are mediated through cytokine and growth factor activities, as well as its ability to bind zinc ions and proteases.

Importance

TIMP1 is relevant to: - Tissue remodelling and repair due to its role in regulating MMP activity, which is crucial for extracellular matrix turnover - Inflammatory responses, as it is involved in connective tissue replacement and wound healing - Cancer biology, since altered TIMP1 expression can influence tumour progression and metastasis - Cardiovascular diseases, where it may impact processes like fibrosis and vascular remodelling

Top Products

For researchers investigating TIMP1, we recommend two excellent primary antibodies. The first is the well-cited polyclonal antibody, Anti-TIMP1 antibody (ab61224), which has garnered 70 citations, reflecting its reliability in Western blotting (WB). Additionally, we offer the recombinant antibody, Anti-TIMP1 antibody [EPR18352] (ab211926), which has been validated in knockout models and is suitable for a broader range of applications, including WB, immunohistochemistry (IHC), and immunocytochemistry (ICC). With 56 citations, this recombinant product ensures batch-to-batch consistency, making it an ideal choice for researchers seeking dependable TIMP1 detection. "The Human TIMP1 ELISA Kit (ab187394), supported by 11 citations, is an excellent option for researchers looking to accurately measure TIMP1 levels in their samples."

Abcam Product Citation Summary

The data indicates that TIMP1 is being studied in various human contexts, particularly in relation to cancer, including gastric and colorectal cancers, as well as glioblastoma. Additionally, TIMP1 is being investigated in mouse models for atherosclerosis and in rat chondrocytes, highlighting its relevance in both cancer research and tissue regeneration studies.

Abcam Product Citation Table

Product Code
Species
Application
Study Context
PMID
ab109125
Human
WB
Collagen deposition
27680712
ab109125
Human
WB
Angiogenesis-related proteins
33064893
ab211926
Human
WB
Colorectal cancer growth and migration
39118103
ab211926
Human
WB
Glioblastoma
35778451
ab38978
Mouse
WB
Atherosclerotic vulnerable plaque formation
31757932
ab61224
Rat
WB
MMP-mediated proteolytic cleavage of type II collagen
29333456
ab61224
Rat
WB
Effects of low-intensity pulsed ultrasound
32477144

Function

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.

Post-translational modifications

The activity of TIMP1 is dependent on the presence of disulfide bonds.

N-glycosylated.

Sequence Similarities

Belongs to the protease inhibitor I35 (TIMP) family.

Tissue Specificity

Detected in rheumatoid synovial fluid (at protein level).

Cellular localization

Alternative names

CLGI, TIMP, TIMP1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Tissue inhibitor of metalloproteinases 1, EPA, Collagenase inhibitor, TIMP-1

swissprot:P01033 omim:151675 entrezGene:7076 entrezGene:3933 entrezGene:4318 swissprot:P31025 swissprot:P14780 omim:305370 omim:120361