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Function

The TNF protein, primarily secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces cell death in specific tumor cell lines and acts as a potent pyrogen, causing fever directly or by stimulating interleukin-1 secretion. TNF is implicated in cachexia induction and can stimulate cell proliferation and differentiation under certain conditions. It impairs regulatory T-cells (Treg) function in rheumatoid arthritis patients through FOXP3 dephosphorylation, upregulating protein phosphatase 1 (PP1), which dephosphorylates 'Ser-418' of FOXP3, inactivating FOXP3 and leading to defective Treg cells. TNF is a key mediator of cell death in the anticancer effect of BCG-stimulated neutrophils with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. It induces insulin resistance in adipocytes by inhibiting insulin-induced IRS1 tyrosine phosphorylation and glucose uptake. TNF plays a role in angiogenesis by inducing VEGF production with IL1B and IL6. Additionally, the TNF intracellular domain (ICD) form stimulates IL12 production in dendritic cells. This supplementary information is collated from multiple sources and compiled automatically.

Involvement in disease

Psoriatic arthritis

PSORAS

An inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoid like pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).

None

Disease susceptibility is associated with variants affecting the gene represented in this entry.

Post-translational modifications

The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.

The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.

O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

Tumor necrosis factor, soluble form

The soluble form is demyristoylated at Lys-19 and Lys-20 by SIRT6, promoting its secretion.

Sequence similarities

Belongs to the tumor necrosis factor family.

Cellular localization

  • Cell membrane
  • Single-pass type II membrane protein
  • Tumor necrosis factor, membrane form
  • Membrane
  • Single-pass type II membrane protein
  • Tumor necrosis factor, soluble form
  • Secreted
  • C-domain 1
  • Secreted
  • C-domain 2
  • Secreted

Alternative names

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

Target type

Proteins

Primary research area

Immunology & Infectious Disease

Other research areas

  • Cardiovascular
  • Immuno-oncology
  • Neuroscience
  • Oncology

Molecular weight

25644Da

We found 87 products in 5 categories

Search our catalogue for 'Tnf' (87)

Products

ab183218

Anti-TNF alpha antibody [EPR19147]

Lab Essentials
Recombinant
RabMAb
KO Validated

ab215188

Anti-TNF alpha antibody [EPR20972]

Lab Essentials
Recombinant
RabMAb
KO Validated

ab255275

Anti-TNF alpha antibody [EPR22598-212]

Lab Essentials
Recombinant
RabMAb
KO Validated

ab307164

Anti-TNF alpha antibody [RM1005]

Lab Essentials
Recombinant
RabMAb
20ul selling size

ab181421

Human TNF alpha ELISA Kit

Recombinant
SimpleStep
KO Validated

ab6671

Anti-TNF alpha antibody

Lab Essentials

ab270264

Anti-TNF alpha antibody [TNF/1500R]

Lab Essentials
Recombinant

ab66579

Anti-TNF alpha antibody

Lab Essentials

ab9579

Anti-TNF alpha antibody [P/T2]

Lab Essentials

ab271939

Anti-TNF alpha antibody [EPR19147] - BSA and Azide free

Recombinant
RabMAb
KO Validated

ab225576

Anti-TNF alpha antibody [EPR20972] - BSA and Azide free

Recombinant
RabMAb
KO Validated