TNNI3
GeneName
TNNI3
Summary
TNNI3, also known as cardiac troponin I, is a 24 kDa protein that is a key component of the cardiac troponin complex, which is located within the cardiac myofibril and sarcomere. It plays a critical role in regulating cardiac muscle contraction by inhibiting the interaction between actin and myosin in the presence of calcium ions. TNNI3 is involved in various processes such as heart development, intracellular calcium ion homeostasis, and muscle filament sliding, and it binds to troponin C and troponin T, contributing to the overall function of the troponin complex in cardiac and skeletal muscle tissues.
Importance
TNNI3 is relevant to: - Heart disease research, particularly in the context of cardiac dysfunction and heart failure due to its role in muscle contraction regulation - Biomarker development for myocardial injury, as elevated levels of cardiac troponin I are indicative of cardiac events - Studies on calcium signalling and its effects on muscle contraction, which are crucial for understanding arrhythmias and other cardiac conditions - Investigations into muscle development and regeneration, which have implications for therapies in muscular dystrophies and other muscle-related disorders
Top Products
For researchers investigating TNNI3, we recommend two excellent primary antibodies. The first is the well-cited polyclonal antibody, Anti-Cardiac Troponin I antibody (ab47003), which has garnered 168 citations, reflecting its strong reputation in the field. This antibody is highly effective for Western blotting (WB), immunohistochemistry (IHC), and immunocytochemistry (ICC). Additionally, we offer the recombinant antibody, Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862). This product is validated for a broader range of applications, including WB, IHC, flow cytometry (FC), and immunoprecipitation (IP). With 21 citations, it is gaining traction among researchers who appreciate the consistency and reliability that recombinant antibodies provide. Together, these antibodies represent a robust toolkit for studying TNNI3. The Human Cardiac Troponin I ELISA Kit (ab229404) is an excellent option for researchers looking to measure TNNI3 levels in their samples.
Abcam Product Citation Summary
The research involving TNNI3 primarily focuses on cardiac contexts, including studies on myocarditis, myocardial infarction, and cardiac myocyte responses to various treatments. The use of different applications such as Western blot and immunohistochemistry highlights the importance of TNNI3 in understanding cardiac function and pathology.
Abcam Product Citation Table
Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Involvement in disease
Cardiomyopathy, familial hypertrophic, 7
CMH7
A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
None
The disease is caused by variants affecting the gene represented in this entry.
Cardiomyopathy, familial restrictive 1
RCM1
A heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
None
The disease is caused by variants affecting the gene represented in this entry.
Cardiomyopathy, dilated, 2A
CMD2A
A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
None
The disease is caused by variants affecting the gene represented in this entry.
Cardiomyopathy, dilated, 1FF
CMD1FF
A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
None
The disease is caused by variants affecting the gene represented in this entry.
Post-translational modifications
Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).
Sequence Similarities
Belongs to the troponin I family.
Alternative names
TNNC1, TNNI3, Cardiac troponin I