TNPO1
GeneName
TNPO1
Summary
TNPO1, also known as MIP, MIP-1, or TRN, is a 102 kDa protein that functions primarily as a nuclear import receptor. It is involved in the transport of proteins into the nucleus by recognising nuclear localisation signals. TNPO1 is expressed in various cellular compartments, including the cytoplasm, nucleus, nucleolus, and cilia, and plays a role in RNA binding and small GTPase interactions. Its localisation to both the cytoplasm and nucleus highlights its dual role in cellular transport processes.
Importance
TNPO1 is relevant to: - Understanding nuclear transport mechanisms, which are essential for regulating gene expression and cellular function - Investigating viral infections, as many viruses exploit nuclear import pathways for replication - Studying cancer biology, given its role in the transport of oncogenic proteins into the nucleus - Exploring neurodegenerative diseases, where nuclear transport dysfunction may contribute to pathology
Top Products
For researchers investigating TNPO1, we recommend two primary antibodies that cater to a variety of applications. The first is the well-cited monoclonal antibody, Anti-Transportin 1/MIP antibody [D45] (ab10303), which has garnered 37 citations, reflecting its reliability in Western blotting (WB), immunocytochemistry (ICC), immunohistochemistry (IHC), and flow cytometry (FC). This antibody is a trusted choice for those requiring robust performance across multiple techniques. Additionally, we offer the recombinant antibody, Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273). While it has not yet accumulated citations, it is validated for use in WB, ICC, and immunoprecipitation (IP). The recombinant nature of this antibody ensures batch-to-batch consistency, making it an excellent option for researchers seeking dependable results in their studies of TNPO1.
Abcam Product Citation Summary
The data indicates that the TNPO1 antibody (ab10303) has been effectively used in various applications, including immunocytochemistry, western blotting, and immunoprecipitation, specifically in human cells and cell lines. The studies focus on protein interactions and circadian rhythms, highlighting the relevance of TNPO1 in these biological contexts.
Abcam Product Citation Table
Function
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed:24753571). May mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5 (PubMed:11682607). In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones (By similarity). In vitro, mediates nuclear import of SRP19 (PubMed:11682607). Mediates nuclear import of ADAR/ADAR1 isoform 1 and isoform 5 in a RanGTP-dependent manner (PubMed:19124606, PubMed:24753571). Main mediator of PR-DUB complex component BAP1 nuclear import; acts redundantly with the karyopherins KPNA1 and KPNA2 (PubMed:35446349).
(Microbial infection) In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
Sequence Similarities
Belongs to the importin beta family. Importin beta-2 subfamily.
Cellular localization
- Cytoplasm
- Nucleus
Alternative names
KPNB2, MIP1, TRN, TNPO1, Transportin-1, Importin beta-2, Karyopherin beta-2, M9 region interaction protein, MIP