TRUB1
Function
Pseudouridine synthase that catalyzes pseudouridylation of mRNAs and tRNAs (PubMed:28073919, PubMed:31477916, PubMed:32926445). Mediates pseudouridylation of mRNAs with the consensus sequence 5'-GUUCNANNC-3', harboring a stem-loop structure (PubMed:28073919, PubMed:31477916). Constitutes the major pseudouridine synthase acting on mRNAs (PubMed:28073919). Also catalyzes pseudouridylation of some tRNAs, including synthesis of pseudouridine(55) from uracil-55, in the psi GC loop of a subset of tRNAs (PubMed:32926445, PubMed:33023933). Promotes the processing of pri-let-7 microRNAs (pri-miRNAs) independently of its RNA pseudouridylate synthase activity (PubMed:32926445). Acts by binding to the stem-loop structure on pri-let-7, preventing LIN28-binding (LIN28A and/or LIN28B), thereby enhancing the interaction between pri-let-7 and the microprocessor DGCR8, which mediates miRNA maturation (PubMed:32926445).
Sequence Similarities
Belongs to the pseudouridine synthase TruB family.
Tissue Specificity
Highly expressed in heart, skeletal muscle and liver. Expressed at lower levels in lung, small intestine, kidney and spleen.
Cellular localization
- Nucleus
- Cytoplasm
- Cytosol
- Catalyzes pseudouridylation of mRNAs in the nucleus.
Alternative names
PUS4, TRUB1, Pseudouridylate synthase TRUB1, TruB pseudouridine synthase homolog 1, tRNA pseudouridine 55 synthase TRUB1, Psi55 synthase TRUB1