Tsg101
Developmental stage
Expressed at all stages of mammary gland development, but at lower rate at early and mid pregnancy. Expressed in 1-cell and 2-cell stage embryos.
Domain
The UEV domain is required for the interaction of the complex with ubiquitin.
The coiled coil domain may interact with stathmin.
Function
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (By similarity).
Post-translational modifications
Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
Sequence Similarities
Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
Tissue Specificity
Ubiquitous. Higher expression in brain and mammary gland. Lower expression in liver and tumoral tissues.
Cellular localization
- Cytoplasm
- Early endosome membrane
- Peripheral membrane protein
- Cytoplasmic side
- Late endosome membrane
- Peripheral membrane protein
- Cytoplasm
- Cytoskeleton
- Microtubule organizing center
- Centrosome
- Midbody
- Midbody ring
- Nucleus
- Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Nuclear localization is cell cycle-dependent. Interaction with CEP55 is required for localization to the midbody during cytokinesis.
Alternative names
Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, TSG101