UBE2M
Domain
Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
Function
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
Pathway
Protein modification; protein neddylation.
Post-translational modifications
The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.
Sequence Similarities
Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
Alternative names
UBC12, UBE2M, NEDD8-conjugating enzyme Ubc12, NEDD8 carrier protein, Ubiquitin-conjugating enzyme E2 M