UBE2O
Function
E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853).
Pathway
Protein modification; protein ubiquitination.
Post-translational modifications
Phosphorylated. Phosphorylation affects subcellular location.
Ubiquitinated: autoubiquitinates, possibly affecting its subcellular location.
Sequence Similarities
Belongs to the ubiquitin-conjugating enzyme family.
Tissue Specificity
Predominantly expressed in skeletal muscle and heart.
Cellular localization
- Cytoplasm
- Nucleus
- Mainly localizes to the cytoplasm.
Alternative names
KIAA1734, UBE2O, (E3-independent) E2 ubiquitin-conjugating enzyme, E2/E3 hybrid ubiquitin-protein ligase UBE2O, Ubiquitin carrier protein O, Ubiquitin-conjugating enzyme E2 O, Ubiquitin-conjugating enzyme E2 of 230 kDa, Ubiquitin-protein ligase O, Ubiquitin-conjugating enzyme E2-230K