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Domain

The YDG domain recognizes and binds 5-hydroxymethylcytosine (5hmC).

Function

E3 ubiquitin ligase that plays important roles in DNA methylation, histone modifications, cell cycle and DNA repair (PubMed:15178429, PubMed:23404503, PubMed:27743347, PubMed:29506131). Acts as a specific reader for 5-hydroxymethylcytosine (5hmC) and thereby recruits various substrates to these sites to ubiquitinate them (PubMed:24813944, PubMed:27129234). This activity also allows the maintenance of 5mC levels at specific genomic loci and regulates neuron-related gene expression (By similarity). Participates in cell cycle regulation by ubiquitinating cyclins CCND1 and CCNE1 and thereby inducing G1 arrest (PubMed:15178429, PubMed:15361834, PubMed:21952639). Ubiquitinates also PCNP leading to its degradation by the proteasome (PubMed:12176013, PubMed:14741369). Plays an active role in DNA damage repair by ubiquitinating p21/CDKN1A leading to its proteasomal degradation (PubMed:29923055). Promotes also DNA repair by acting as an interstrand cross-links (ICLs) sensor. Mechanistically, cooperates with UHRF1 to ensure recruitment of FANCD2 to ICLs, leading to FANCD2 monoubiquitination and subsequent activation (PubMed:30335751). Contributes to UV-induced DNA damage response by physically interacting with ATR in response to irradiation, thereby promoting ATR activation (PubMed:33848395).

Involvement in disease

Associated with various cancers. DNA copy number loss is found in multiple kinds of malignancies originating from the brain, breast, stomach, kidney, hematopoietic tissue and lung.

Pathway

Protein modification; protein ubiquitination.

Post-translational modifications

May be autoubiquitinated; which may lead to proteasomal degradation.

Phosphorylated. Phosphorylation may be mediated by CDK2.

Autosumoylated.

Cellular localization

  • Nucleus
  • Chromosome
  • Enriched at genomic loci that are enriched for 5-hydroxymethylcytosine (5hmC).

Alternative names

NIRF, RNF107, UHRF2, E3 ubiquitin-protein ligase UHRF2, Np95/ICBP90-like RING finger protein, Nuclear protein 97, Nuclear zinc finger protein Np97, RING finger protein 107, RING-type E3 ubiquitin transferase UHRF2, Ubiquitin-like PHD and RING finger domain-containing protein 2, Ubiquitin-like-containing PHD and RING finger domains protein 2, Np95-like RING finger protein

Target type

Proteins

Primary research area

Epigenetics

Molecular weight

89985Da

We found 1 product in 1 category

Primary Antibodies

Target

Application

Reactive species

Search our catalogue for 'NIRF' (1)

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