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VDR

GeneName

VDR

Summary

VDR, also known as the vitamin D receptor or hVDR, is a 48kDa nuclear receptor that plays a crucial role in mediating the effects of vitamin D. It is primarily expressed in various tissues, including the intestine, bone, and immune cells. VDR functions as a transcription factor, binding to specific DNA sequences to regulate the expression of genes involved in calcium and phosphate homeostasis, bone metabolism, and cell differentiation. It is located in the nucleus and cytoplasm, and is part of several complexes including the RNA polymerase II transcription regulator complex. VDR is also involved in various biological processes such as intestinal absorption, mammary gland development, and regulation of gene expression.

Importance

VDR is relevant to: - Bone health and metabolic bone diseases, given its role in calcium and phosphate homeostasis and bone mineralization - Immune function, as it influences the activity of immune cells and their response to infections - Cancer research, particularly in breast and colon cancers, where vitamin D signalling may impact cell proliferation and differentiation - Disorders related to vitamin D deficiency, which can lead to conditions such as rickets and osteomalacia - The regulation of gene expression in response to environmental factors, including dietary components like bile acids and retinoids

Top Products

For researchers investigating the Vitamin D Receptor (VDR), we recommend two excellent primary antibodies. The first is the well-cited polyclonal antibody, Anti-Vitamin D Receptor antibody (ab3508), which has garnered 79 citations and is particularly effective for immunohistochemistry (IHC). This antibody is a trusted choice for those looking to study VDR in tissue samples.In addition, we offer the recombinant antibody, Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234). This monoclonal antibody has been validated in knockout models and is suitable for a variety of applications, including Western blotting (WB), chromatin immunoprecipitation (ChIP), and immunoprecipitation (IP). With 50 citations, it demonstrates a solid reputation in the research community. This combination of antibodies provides a comprehensive toolkit for VDR research, catering to both tissue and cellular studies.

Abcam Product Citation Summary

The data indicates that VDR antibodies from Abcam have been extensively used in various studies involving both human and mouse models. The applications primarily include Western blotting and ChIP assays, focusing on cellular responses to vitamin D3 and its effects on differentiation, aging, and oxidative stress. This highlights the importance of VDR in metabolic and physiological processes.

Abcam Product Citation Table

Product Code
Species
Application
Study Context
PMID
ab109234
Rat
WB
Glucose modulation
29416624
ab3508
Human
WB
Myeloid cell lines
24263100
ab3508
Human
WB
HL60 and MonoMac-6 cell lines differentiation
24263100
ab3508
Human
WB
Monocyte differentiation
24263100
ab3508
Mouse
ChIP
Cellular senescence
31880094
ab3508
Mouse
WB
Physiological aging
35906886
ab3508
Mouse
ChIP
Oxidative stress and DNA damage
30907059

Domain

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Function

Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells (PubMed:10678179, PubMed:15728261, PubMed:16913708, PubMed:28698609, PubMed:37478846). Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR (PubMed:28698609). The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes (PubMed:28698609). Plays a central role in calcium homeostasis (By similarity). Also functions as a receptor for the secondary bile acid lithocholic acid (LCA) and its metabolites (PubMed:12016314, PubMed:32354638).

Involvement in disease

Rickets vitamin D-dependent 2A

VDDR2A

A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.

None

The disease is caused by variants affecting the gene represented in this entry.

Post-translational modifications

Ubiquitinated by UBR5, leading to its degradation: UBR5 specifically recognizes and binds ligand-bound VDR when it is not associated with coactivators (NCOAs) (PubMed:37478846). In presence of NCOAs, the UBR5-degron is not accessible, preventing its ubiquitination and degradation (PubMed:37478846).

Sequence Similarities

Belongs to the nuclear hormone receptor family. NR1 subfamily.

Cellular localization

Alternative names

NR1I1, VDR, Vitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1

swissprot:P11473 omim:601769 entrezGene:7421

Other research areas