WIPI2 phospho S413
Domain
The L/FRRG motif is required for recruitment to PtdIns3P.
Function
Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Involved in an early step of the formation of preautophagosomal structures. Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases (PubMed:23051912, PubMed:23291478, PubMed:24954904). Mediates ER-isolation membranes contacts by interacting with the ULK1:RB1CC1 complex and PtdIns3P (By similarity). Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane.
Sequence Similarities
Belongs to the WD repeat PROPPIN family.
Cellular localization
- Preautophagosomal structure membrane
- Peripheral membrane protein
- Cytoplasmic side
- Localizes to omegasomes membranes which are endoplasmic reticulum connected structures at the origin of preautophagosomal structures. Enriched at preautophagosomal structure membranes in response to PtdIns3P.
Alternative names
WD repeat domain phosphoinositide-interacting protein 2, WIPI-2, Wipi2