ZEB1
GeneName
ZEB1
Summary
ZEB1, also known as BZP, ZEB-1, or TCF8, is a 124 kDa transcription factor that plays a pivotal role in various developmental processes and cellular differentiation. It is primarily located in the nucleus, where it binds to chromatin and acts as a transcriptional repressor or activator, depending on the context. ZEB1 is involved in the regulation of gene expression through its interactions with DNA, particularly at E-box elements, and is known to bind zinc ions. This protein is crucial for cartilage development, central nervous system development, and the differentiation of various cell types, including T cells and smooth muscle cells. Additionally, ZEB1 has roles in keratinocyte proliferation and the morphogenesis of structures such as the cochlea and the embryonic skeletal system.
Importance
ZEB1 is relevant to: - Cancer research, particularly in the context of epithelial-mesenchymal transition (EMT), which is associated with metastasis and tumour progression - Developmental biology, as it regulates key processes such as organogenesis and tissue differentiation - Neurobiology, given its involvement in central nervous system development and neuron differentiation - Regenerative medicine, due to its role in cell differentiation and proliferation, which could inform strategies for tissue repair and regeneration
Top Products
For researchers investigating ZEB1, we highly recommend the top-selling recombinant antibody, Anti-ZEB1 antibody [EPR17375] (ab203829). This well-cited product has garnered 151 citations, reflecting its strong reputation in the field. It has been validated in knockout models and is suitable for a variety of applications, including Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), and flow cytometry (FC). The versatility and reliability of this antibody make it an excellent choice for those studying ZEB1.
Abcam Product Citation Summary
The data indicates a significant focus on ZEB1 in various human cancer contexts, particularly in studies related to epithelial-mesenchymal transition (EMT) and breast cancer metastasis. The use of multiple antibodies in Western blotting highlights the importance of ZEB1 in understanding cancer progression and treatment resistance. Additionally, there are studies involving ZEB1 in rat models, suggesting its relevance in broader biological contexts.
Abcam Product Citation Table
Function
Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region (By similarity).
Involvement in disease
Corneal dystrophy, posterior polymorphous, 3
PPCD3
A subtype of posterior corneal dystrophy, a disease characterized by alterations of Descemet membrane presenting as vesicles, opacities or band-like lesions on slit-lamp examination and specular microscopy. Affected patient typically are asymptomatic.
None
The disease is caused by variants affecting the gene represented in this entry.
Corneal dystrophy, Fuchs endothelial, 6
FECD6
A corneal disease caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition.
None
The disease is caused by variants affecting the gene represented in this entry.
Post-translational modifications
Ubiquitinated, leading to degradation in a proteasome-dependent manner. Deubiquitinated by USP51, leading to stabilization.
Sequence Similarities
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
Tissue Specificity
Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.
Cellular localization
- Nucleus
Alternative names
AREB6, TCF8, ZEB1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8
Database links
swissprot:P37275 omim:189909 entrezGene:6935
Other research areas
- Oncology