ZNF207
Domain
The GLEBS region mediates interaction with BUB3 (PubMed:24462186, PubMed:24462187).
The microtubule-binding region is required for efficient loading of BUB3 onto kinetochores and proper mitosis.
Mainly composed of disordered low-complexity regions outside of the C2H2-type zinc fingers. Coacervation depends on hydrophobic and aromatic Phe and Tyr in the disordered low-complexity region, that may promote coacervation by forming intermolecular hydrophobic interactions.
Function
Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly (PubMed:24462186, PubMed:24462187, PubMed:26388440). ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks (PubMed:26388440). Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3 (PubMed:24462186, PubMed:24462187). Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3 (PubMed:24462186). According to another report, the stabilization is independent of the proteasome (PubMed:24462187).
Tissue Specificity
Ubiquitous.
Cellular localization
- Nucleus
- Chromosome
- Centromere
- Kinetochore
- Cytoplasm
- Cytoskeleton
- Spindle
- Localizes primarily to the nucleus in interphase, concentrates at kinetochores prior to nuclear envelope breakdown and during early prometaphase, and disappears from kinetochores upon microtubule-binding.
Alternative names
BUGZ, ZNF207, BUB3-interacting and GLEBS motif-containing protein ZNF207, BuGZ, hBuGZ, Zinc finger protein 207