zwf
Function
Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.
Probable source of extracellular death factor (EDF, sequence Asn-Asn-Trp-Asn-Asn, NNWNN) following processing and amidation. This pentapeptide stimulates cell death mediated by MazF (PubMed:17962566). Artificial peptides with altered sequence show that NNGNN, GNWNG and NWN no longer stimulate MazF's endoribonuclease activity; other peptides (NNGN, GNWMM, NNWNG, NNNWNNN) retain MazF-stimulating activity. NNWNN, NNGN, GNWMM and NNWNG prevent cognate antitoxin MazE from inhibiting MazF; although NNNWNNN stimulates MazF it does not do so in the presence of MazE. EDF also stimulates ChpB's endoribonuclease activity in vitro; in this case NWN partially stimulates ChpB, whereas NNGNN, GNWNN, NNWNG, GNWNG and NNNWNNN do not. Only the wild-type EDF peptide prevents cognate antitoxin ChpS from inhibiting ChpB (PubMed:21419338).
Pathway
Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Post-translational modifications
Probably processed by the ClpPX protease to generate the extracellular death factor (EDF). It is thought that processing produces Asn-Asn-Trp-Asp-Asn which is amidated to generate Asn-Asn-Trp-Asn-Asn (Probable).
Sequence Similarities
Belongs to the glucose-6-phosphate dehydrogenase family.
Alternative names
b1852, JW1841, Glucose-6-phosphate 1-dehydrogenase, G6PD