Protocols for Cell culture

This protocol will guide you through isolating immature bone marrow-derived dendritic cells (BMDCs) from mice.

Cells require careful maintenance to prevent contamination, facilitate growth and ensure long-term stability. The following pages provide general guidance for preserving, thawing and maintaining cultured cells. Note: specific procedures will be highly dependent on the cell line used, and you should refer to the cell line providers for detailed advice.

View our detailed step-by-step protocol explaining how to obtain a viable cell count from a hemocytometer.

Learn about cryopreservation of mammalian cell lines for long term storage in liquid nitrogen.

This protocol outlines how to chemically induce the differentiation of 3T3-L1 cells into adipocyte-like cells, adapted from Reed & Lane (1980).

Procedure for biological and chemical induction of apoptosis in cells.

This protocol describes the isolation of human platelets from whole blood.

Find out the procedure for RNA isolation, DNase treatment and reverse transcription in our detailed protocol for cells in culture and tissue samples.