ChIC/CUT&RUN-seq

ChIC/CUT&RUN sequencing, short for Chromatin Immuno-Cleavage/Cleavage Under Targets and Release Using Nuclease, is an innovative technique for chromatin profiling. This method combines the principles of chromatin immunocleavage (ChIC) and CUT&RUN to analyze DNA-protein interactions with high precision. Unlike traditional ChIP-seq, which relies on sonication to fragment the chromatin, ChIC/CUT&RUN uses pAG-MNase to cleave DNA at protein binding sites selectively. The process involves binding cells to magnetic beads, targeting specific proteins with antibodies, and using the pAG-MNase enzyme to cut the DNA at these sites. The cleaved DNA fragments are then released, purified, and sequenced to determine their genomic locations. This technique offers several advantages over ChIP-seq, including lower background noise, reduced cell requirements, and faster processing times.

ChIC/CUT&RUN is particularly useful for studying transcription factor binding, histone modifications, and other chromatin-associated proteins. Its high sensitivity and specificity make it ideal for analyzing rare or precious samples, such as primary tissues and sorted cells. This method has broad applications in epigenetics, gene regulation research, and the study of various diseases.

Working with top scientists in the field, we developed these up-to-date ChIC/CUT&RUN-seq resources including step-by-step protocols to aid your epigenetics research.