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Protocols for ELISA

Protocols to help you run your ELISA experiments.

Clear, comprehensive, and up-to-date protocols from our experts and collaborators so you can run your ELISA experiments with consistent, accurate results.

  • Direct ELISA using fluorescent substrate protocol

    Procedure and tips for direct ELISA assay using a fluorescent conjugated primary antibody.

  • In-cell ELISA protocol

    In-Cell ELISA (also known as cell-based ELISA, in cell western or cytoblot) is an immunocytochemistry method used to quantify target protein or post-translational modifications of the target protein, in cultured cells.

  • Indirect and Direct ELISA

    Our protocol for traditional ELISA formats using indirect and direct detection.

  • Sandwich ELISA protocol

    Learn how to set up a sandwich ELISA, covering all steps from plate coating and blocking to incubations with primary and secondary antibodies.

  • ELISPOT protocol

    General ELISPOT procedure outlining plate and sample preparation, cell incubation, enzymatic detection, and analysis.