Flow cytometry
All our resources and tools for flow cytometry.
Accelerate immunometabolism flow discovery
Get deeper insights from rare populations with a unique portfolio of flow-validated antibodies to 90% of key metabolic enzymes. Drop-in ready for rapid panel expansion.
Panel builder
Create optimized panels tailored to your instrument and experimental needs with our new interactive tool, powered by FluoroFinder.
Spectra viewer
Explore our interactive spectra viewer and build reproducible, high-resolution panels.
Flow cytometry solutions
In research and clinical settings, flow cytometry is widely used for immunophenotyping, assessing the cellular composition of samples, evaluating cellular function and health, isolating selected cells by sorting, and in biomarker and drug discovery.
Explore our curated catalog of conjugated and carrier-free antibodies, conjugation and assay kits, and more, to find solutions to support your research.
Cell and gene therapy solutions
Accelerate your cell and gene therapy discovery workflow with our comprehensive collection of high-quality assays and reagents.
Tools for neutrophil depletion and analysis
Unlock new insights into immune function with our range of neutrophil depletion kits, specific antibodies, and advanced detection assays.
Our top flow cytometry resources
Panel builder and spectra viewer
Create optimized panels and view high-resolution spectra with our interactive tools.
Flow cytometry protocols and best practices
Optimize your workflow with our flow cytometry protocols across all staining types and detection systems.
Flow cytometry troubleshooting
We’ve asked our flow cytometry experts to put together their best explanations and solutions for common issues like no fluorescent signal, low event rate, and more.
The complete flow cytometry guide
Flow cytometry is a popular laser-based technology to analyze the characteristics of cells or particles. Discover more with our introduction to flow cytometry.
Guide on the basics of fluorescence
Understand fluorescence and how it enhances scientific exploration.
Get fully equipped to run your flow cytometry experiments. Our free comprehensive course features guidance on the flow cytometer, sample preparation, compensation and data analysis, and troubleshooting.
A quick and easy guide that helps you select the most appropriate fluorochrome from the most popular labels.
Explore essential flow cytometry buffers, reagents, and equipment, including compensation beads, Fc blocking buffers, fixation buffers, fix‑and‑perm solutions, and isotype controls.
Panel design and fluorescent dyes
Explore essential tools and guidance for building high‑performance flow cytometry panels, including an interactive panel builder, real‑time spectra viewer, best‑practice guides for conventional and spectral cytometry, a comprehensive fluorochrome chart, and a full fluorescent dyes guide to support accurate, efficient multicolor experiment design.
Flow cytometry cell viability dyes
Use our selection guide to browse easy-to-use kits and eliminate dead cells from your flow cytometry data analysis.
Immunophenotyping resources
Marker guides
Posters
Flow cytometry is a powerful technique that analyzes the physical and chemical characteristics of cells and particles as they flow in a fluid stream through a beam of light. By measuring parameters like cell size, granularity, and fluorescence intensity, flow cytometry provides detailed insights into cell populations and their functions.
Explore our curated content to enhance your flow cytometry experiments and move your research forward. Our resources are designed to help you master the intricacies of flow cytometry, from setting up your experiments to interpreting complex data.
Our flow cytometry guide gives you everything you need to run your flow cytometry experiments from start to finish, including multicolor flow cytometry. Our protocols will help you optimize your flow cytometry workflow, and our troubleshooting tips give you expert advice on how to resolve common flow cytometry snags. Our product recommendations will help you easily find the very best reagents for your flow cytometry experiments, including fluorescent conjugated primary antibodies, carrier-free antibodies, conjugation kits and assay kits. Explore our flow cytometry resources and get the tools you need to move your research forward.
Immunophenotyping by flow cytometry
Learn how to design effective panels and avoid common pitfalls in immunophenotyping by flow cytometry.
Is DAPI flow cytometry still relevant in the age of high-dimensional analysis?
Let’s break down where DAPI flow cytometry fits into today’s workflows, when it might hold you back, and how it stacks up against newer alternatives.
The story of spectral flow cytometry, told by one of its creators
Dr J. Paul Robinson is a Distinguished Professor of Cytometry at Purdue University and a pioneer of spectral flow cytometry, a revolutional technology.
Here, Paul shares some personal recollections from his career journey and explains how the first spectral flow cytometer was developed with inspiration from Landsat technology, despite doubts that it would ever really work.
