Staining
Staining with dyes and chemical agents is an essential method for studying biological samples. This technique enhances the visibility of cells, tissues, and microorganisms, allowing you to observe and analyze their structure, composition, and function more clearly.
We’ve collated resources dedicated to various staining methods and applications. From basic histological stains to fluorescent dyes, we cover a wide range of techniques to help you achieve accurate, reproducible results. Explore detailed protocols covering Fura-2 AM imaging, Annexin V-FITC staining, BRDY staining, Phalloidin staining and more. Whether you’re working with tissue sections, cell cultures or microbial samples, our curated content will guide you through selecting and optimizing staining procedures.
Protocols for Staining
Here, you’ll find all the resources you need, from principles to protocols and analysis.
Annexin V-FITC staining for detecting apoptosis
Procedure for the early detection of apoptosis using annexin V-FITC staining and optional propidium iodide (PI).
Paul Polak and Douglas Feinstein, University of Illinois, Chicago. Bielschowsky's silver stain is a very useful tool to detect nerve fibers. It can be used to stain axons, neurofibrils and senile plaques in the central nervous system. This method is easy to perform and is routinely used in the study of Alzheimer's disease together with antibody staining.
Protocol for identifying proliferating cells using BrdU.
Mouse-on-mouse (MOM) staining protocol
Methods for reducing background when staining mouse tissue with a mouse monoclonal antibody.
Detailled procedure for staining with phalloidin dye conjugates, including tips for choosing the most suitable phalloidin conjugate.