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Protocols for Staining

Clear and comprehensive staining protocols compiled by our experts, to help you deliver consistent and accurate results.
  • Fura-2 AM imaging protocol

    This FURA-2 AM imaging protocol was provided by Prof Amy Harkins, St Louis University.

  • Annexin V-FITC staining for detecting apoptosis

    Procedure for the early detection of apoptosis using annexin V-FITC staining and optional propidium iodide (PI).

  • Bielschowsky's silver stain

    Paul Polak and Douglas Feinstein, University of Illinois, Chicago. Bielschowsky's silver stain is a very useful tool to detect nerve fibers. It can be used to stain axons, neurofibrils and senile plaques in the central nervous system. This method is easy to perform and is routinely used in the study of Alzheimer's disease together with antibody staining.

  • BRDU staining

    Protocol for identifying proliferating cells using BrdU.

  • Mouse-on-mouse (MOM) staining protocol

    Methods for reducing background when staining mouse tissue with a mouse monoclonal antibody.

  • Phalloidin staining

    Detailled procedure for staining with phalloidin dye conjugates, including tips for choosing the most suitable phalloidin conjugate.