Western blot
All tools and resources for western blot.
The complete guide to western blot
Our guide breaks down each stage of western blotting, from sample preparation and gel electrophoresis to membrane transfer, blocking, antibody selection, and detection.
Western blot protocols and best practice guides
Clear, comprehensive, and up-to-date western blot protocols, sample preparation guides and best practice guides designed by experts for consistent and reproducible results every time.
Western blot troubleshooting
Need help with your western blot experiment? Find causes and solutions to common problems with our comprehensive troubleshooting resources.
Western blot buffers, reagents and equipment
Explore essential western blot buffers, reagents, and equipment, including guides for Bradford assay, buffer recipes, enhanced chemiluminescence, protein ladders, and best practices for effective western blot blocking.
Dot blot
With protocols for general dot blot, competitive dot blot, and RNA dot blot, you’ll be equipped with the tools you need whatever your workflow.
Western blotting is a widely used technique in research for detecting proteins or antigens in various sample types.
Here, you will find everything you need to elevate your western blotting technique, from detailed protocols, training materials, and guides to in-depth articles covering each aspect of the procedure, as well as our selection of western blotting products. These resources can aid your research regardless of your level, providing you with the necessary knowledge and tools.
We offer a range of products for western blotting, including our large catalog of award-winning primary and secondary antibodies. These include our recombinant monoclonal antibodies, which are extensively validated in numerous applications across several tissue types and disease states and by using knock-out cell lines. Our recombinant monoclonal antibodies provide excellent specificity and sensitivity with minimal batch-to-batch variability. Additionally, our range of secondary antibodies can be used to detect primary antibodies raised in multiple species and come ready conjugated with various reporter molecules for detection.
Recommended western blot resources
Get your western blot experiment right first time with our extensive library of western blotting resources: protocols, troubleshooting guides, webinars and more.
Clear, comprehensive, and up-to-date western blot protocols designed by experts for consistent and reproducible results every time.
Problems, causes and solutions for issues in western blot.
The complete guide to western blot
Western blotting is a technique that uses specific antibodies to identify proteins that have been separated based on size by gel electrophoresis. Plan and optimize your western blot experiments with our guide.
Western blot buffers, reagents and equipment
Explore essential western blot buffers, reagents, and equipment, including guides for Bradford assay, buffer recipes, enhanced chemiluminescence, protein ladders, and best practices for effective western blot blocking.
Webinar: Western blotting at 40
Join Dr. Hanna Dreja for the most recent know-how in western blotting. This technique may be middle-aged, but with being published on PubMed more than 400,000 times, it remains highly relevant. Learn how to get the most from your western blots. The webinar covers all you need from standard protocols and selecting the best reagents, to multiplexing and answers to your FAQs.
90 mins watch
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