Skip to main content

Antibody basics guide

A comprehensive guide to antibody basics: from antibody structure and production to antibody selection and handling.

A comprehensive introduction to antibodies, learn all about primary antibodies and secondary antibodies. Includes detailed descriptions of how they are produced, the different types and structures of antibodies, how to choose the best antibody for your experiments and a glossary of antibody terms and applications.

Antibody basics guide


  • Antibody basics

    Antibodies are large, Y-shaped glycoproteins produced by B-cells as a primary immune defense. Antibodies specifically bind unique molecules of a pathogen, called antigens.

  • An Introduction to antibody production

    Here we review the benefits and limitations of monoclonal, polyclonal, and recombinant monoclonal antibodies and how they are produced.

  • Antibody methods and techniques

    Antibodies are powerful research tools used in various lab techniques. Here we provide a brief overview of the most popular lab techniques, highlighting how they use antibodies.

  • How to choose and use antibodies

    A primary antibody is an antibody that binds directly to a target protein, with a variable antibody region recognizing a protein's epitope. There are a few points to consider when hoosing a primary antibody.

  • Antibody glossary

    A collection of definitions from throughout the guide.