Validating in your lab
Essential checks before starting your experiments.
We recommend that you perform initial in-house validation with your cancer biomarker antibody before you begin your experiments.
- For well-established IHC antibodies, your own in-house validation can be as straightforward as reproducing the expected result for your cancer biomarker antibody on positive and negative tissues to gain an appropriate signal.
- When investigating validated cancer biomarkers in a new tissue or sample preparation, or novel cancer biomarkers, your own validation may include positive control material from previously investigated tissue. Antibody staining and signal consistency should match those from previously validated tissues. This allows you to optimize your antibody for use in your sample of interest.