General considerations
Points to keep in mind when analyzing more than one parameter to identify which type of cell death we see in the studied cell population.
Download our guide on cell death analysis types
General considerations
As we have mentioned throughout this guide, it is recommended to analyze more than one parameter to identify which type of cell death we see in the studied cell population. This is because multiple parameters can be observed in different types of cell death such as necrosis or necroptosis.
Things to keep in mind:
- When choosing parameter to study, ensure that it is relevant to your experimental conditions
- Perform an initial dose response curve to identify the appropriate concentration of a compound and timing of the treatment for best results in your cells of interest
- It is important to minimize the number of processing steps to limit the possibility of losing dying cells
- Published literature and protocols offer invaluable guidance but do not rely on them exclusively to set your experimental condition – Subtle lab-to-lab differences may affect your results