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The complete ELISA guides

Everything you need to know to perform your ELISA experiments in one convenient guide.

Enzyme-linked immunosorbent assay (ELISA) is a widely established technology to detect the presence of antigens in samples. Whether you are considering setting up your own ELISA or use one of our ELISA kits, you will find all the information you need in here. Our complete guide to ELISA takes you from basic ELISA principles to protocols, analysis, and troubleshooting. 

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ELISA application guide


  • What is an ELISA?

    Learn how ELISA assays work, the advantages and disadvantages of this technique, and the different types of ELISA available.

  • Types of ELISA

    Learn about the different types of ELISA available.

  • Choosing how to analyse your ELISA

    Learn the correct way to analyze your ELISA data including advice on standard curve preparation.

  • What ELISA should I choose?

    Choose the best ELISA kit for your sample by looking at CV, % Recovery and linearity.

  • Required controls for ELISA

    Positive or negative? Spike or endogenous? Get the best possible results with your ELISA by using the right control samples with our complete guide.

  • ELISA sample preparation

    Read our tips for preparing samples before running an ELISA in this detailed protocol.

  • Data analysis for ELISA

    Discover our recommended guidelines on statistical assay validation along with calculations of results from ELISA data.

  • Glossary of terms for ELISA

    Definition of the terms commonly used when running or developing ELISA