JavaScript is disabled in your browser. Please enable JavaScript to view this website.

ELISA sample preparation

Read our tips for preparing samples before running an ELISA in this detailed protocol.

ELISA application guide

PDF

Download
button-secondary
icon-none

ELISA sample preparation guide

Please refer to the protocol included with your kit for product-specific details regarding sample preparation and compatible sample types.

These guidelines are intended to be an educational resource for preparing commonly tested samples for use in ELISA assays. Optimum sample preparation procedures will vary depending on the target and assay of interest. When developing a new assay, it is always good practice to consult the literature for experimental examples similar to your own.

Sample preparation methods

Cell culture supernatant

Cell extract

Conditioned medium

Milk

Plasma

Urine

Saliva

Serum

Tissue extract

Tip: The volumes of lysis buffer must be determined in relation to the amount of tissue present. The typical concentration of the final protein extract should be >1 mg/ml.

Cell/tissue extraction buffer recipe

Additional reagents required to produce complete extraction buffer.

Tip: Supplement the cell extraction buffer with phosphate and protease inhibitor cocktails as described by the manufacturer and add PMSF to a final concentration of 1 mM immediately before use.

General recommendations