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Epigenetics application guide

Find out about the key areas of epigenetics including histone modifications, chromatin architecture, DNA and RNA modifications.

Epigenetic regulation occurs on many interacting levels, and it is essential to examine all of these levels in  parallel to understand epigenetic contributions to biological processes. Tackling epigenetic studies from multiple angles with redundancy is key to ensuring accurate results.

Why is epigenetics important? 

Completion of the human genome project and advances in next-generation sequencing technologies have revealed that genomic DNA has much less control over biological processes and disease states than initially thought. Instead, epigenetic factors dictate how DNA is translated, tightly regulating DNA structure to control which genes to express at what times. 

Many of these epigenetic factors work together to orchestrate essential cellular programs, from developmental processes to cell death pathways. Dysfunction of any of these factors can upset genomic regulation, causing cellular processes to go awry, resulting in disease from cancers and autoimmune disorders to neurological conditions, infertility, and everything in between.

To understand any aspect of biology or disease, it is essential to examine epigenetic factors that may contribute.

Epigenetics application guide
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  • Chromatin accessibility and architecture

    Genomic DNA is packaged and organized into chromatin. Understanding which genomic regions are active vs inactive can help to identify critical disease paths.

  • Histone modifications

    Learn about the most common histone modifications eg H3K4me3, H3K9me3, and H3K27me3 and their readers, writers, and erasers.

  • Studying epigenetics using ChIP

    Find out about the different epigenetic factors which require analysis by ChIP and help determine which ChIP method is right for you in our guide to ChIP.

  • Chromatin profiling using ChIC/CUT&RUN

    See how a new chromatin profiling method, ChIC/CUT&RUN, overcomes many of the drawbacks of conventional ChIP methods with our comprehensive guide.

  • DNA methylation and demethylation

    Learn about DNA methylation (5mC) and the mechanisms of DNA demethylation and techniques used to map DNA modifications 5mC, 5hmC, 5fC, and 5caC.

  • RNA modifications

    Learn different applications and techniques for determining the presence and distribution of RNA modifications in mRNA, tRNA and more.